Establishing the Phytoplankton Culture

Establishing the Culture
Add enough media to the culture to double the volume.  Insert a rigid airline and start a moderate flow of bubbles.  Watch the culture over the next few days.  Diluting the culture should make it lighter in color–when the culture returns to the original color, or darker, dilute again by 50% and repeat until the culture volume reaches 1 gallon.  Another popular culturing volume is 2L–you can use clear soda bottles to do that.  The main point here is to take it slow in the beginning, allow your culture to take-hold and help keep possible contamination at bay.  Once your culture has reached a deep green color in the 1 gallon vessel, you are ready to subdivide.

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